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Collection: iBAT Sympathetic Innervation Circuit Pseudorabies Viral Tracing_2019

Study purpose: To identify the location of pre- and post-ganglionic neurons as well as nerves that selectively innervate the interscapular brown adipose tissue, iBAT, in the mouse. Data collection: Pseudorabies virus was injected into interscapular brown adipose tissue, iBAT, and then reporter mice, iDISCO. Light-sheet microscopy and confocal microscopy were used to collect high-resolution image files, qualitative anatomical identification, quantitative cell counts and videos.
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